VAHTS RNA Clean Beads

Number: N412

• SIZE： 5 ml 60 ml 450 ml

The Vazyme VAHTS RNA Clean Beads is based on SPRI (Solid Phase Reverse Immobilization) and is applicable for RNA purification. This kit selectively binds RNA to the beads and efficiently removes all proteins, salt ions, and other impurities.
The usage of VAHTS RNA Clean Beads is the same as the Agencourt® RNAClean® XP Beads (Beckman Coulter, Cat.No. #A63987), which is widely used currently. The cost-effective VAHTS RNA Clean Beads serves as a seamless alternative for Agencourt® RNAClean® XP Beads.

Tip

1. Equilibrate the VAHTS RNA Clean Beads to room temperature before use and suspend the beads thoroughly by vortexing every time before pipetting.
2. Avoid the contamination of RNase and nucleic acid during the experiment.
3. The 80% ethanol should be prepared with nuclease-free water to avoid RNA degradation by RNase.
4. When air-drying the beads, do not over-dry it. Over-dried beads with cracks on the surface will lead to reduced elution efficiency of RNA.
5. IntheprotocolStep10,avoidpipettingbeadswhentransferringthesupernatant,i.e.leavebehind2-3μlofsupernatant.
6. The VAHTS RNA Clean Beads is compatible for various library prep kits, such as VAHTS mRNA-seq v2 for Illumina® (Vazyme, Cat.No. #NR601), VAHTS
   Stranded mRNA-seq for Illumina® (Vazyme, Cat.No. #NR602), and VAHTS Total RNA-seq (H/M/R) for Illumina® (Vazyme, Cat.No. #NR603). Please refer to the protocols of these kits when using the VAHTS RNA Clean Beads for library preparation.

Manual Download

• VAHTS RNA Clean Beads