AquaScreen® FastExtract

The AquaScreen® product line is an in vitro test system for quantitative detection of various water pathogens.

The AquaScreen® FastExtract kit is specifically designed for the extraction of microbial DNA from water samples.

The kit complies with the design requirements of ISO/TS 12869. The extracted DNA can be tested with our AquaScreen® qPCR kits for sensitive and reliable detection of legionella, E. coli and P. aeruginosa in water samples.

The AquaScreen® FastExtract kit works with flexible water sample volumes, using 0.4 µm filter membranes to capture the microorganisms potentially present. Next, the obtained bacterial cells are lysed on the filter membrane and their DNA purified by spin column. The DNA is eluted in small volumes and ready-to-use for qPCR.

Recommended Use

AquaScreen® FastExtract can be used with your established vacuum filtration device (47 mm frit) for the extraction of DNA from legionella and other microbial species. This product is optimized for high throughput and provides high quality DNA for subsequent PCR analysis.

Kit Components

Membrane filters
Incubation dishes
Incubation, collection and sample storage tubes
Spin Columns
Lysis, binding, wash and elution buffers

Package Sizes

• Cat. No. 32-101010 extractions
• Cat. No. 32-105050 extractions

Required Lab Devices & Consumables

Vacuum pump
Filtration system, 47 frit
Microcentrifuge
Pipettes and filter tips
Incubator (37 °C for petri dishes) and heat block (56 °C for reaction tubes)
Ethanol (96-100 %)

Shelf Life and Storage

Components can be stored at room temperature until the expiry date indicated on the label.

Compliance

AFNOR XP T90-471 and ISO/TS 12869 in combination with AquaScreen® qPCR kits

Files to download: