HMW DNA Extraction, RevoluGen, UK

With Fire Monkey and Fire Flower, the long-read DNA sequencing revolution starts with RevoluGen

RevoluGen’s Nucleic Acid Isolation and Purification (NAIP) Fire Monkey kit is the only technology that allows extraction and simultaneous size selection of long-length DNA fragments, which are critical for long-read DNA sequencing.

Improves long-read sequencing results – longer DNA fragment extraction by Fire Monkey technology allows for better structural assembly information

Rapid – Fire Monkey can extract and purify long fragments of DNA within one hour and is ready for immediate use

User-friendly Fire Monkey is a simple spin-column procedure, using standard laboratory equipment

Fire Monkey’s built-in size selection process eliminates one damaging sample handling step post-extraction. This saves time and protects long DNA fragments from further traumatic breaks

Facilitates complete bacterial genome plus full plasmid assembly in a single contig due to the combination of long DNA fragments and high throughput sequencing yields

Validated on Oxford Nanopore, PacBio, and 10X genomics sequencing platforms

Product Image For Research Use Only. Not intended for diagnostic purposes

FIREMONKEY

Fire Monkey extracts high molecular weight DNA from bacterial and mammalian cells within one hour.

Fire Monkey is a rapid and user-friendly spin-column nucleic acid isolation and purification kit. It extracts pure high molecular weight DNA and RNA from a cell sample with an average length of 100,000 base pairs or more, incorporating a simultaneous size depletion function that reduces the amount of smaller DNA fragments present in the final extract.

Longer starting DNA is essential to long-read DNA sequencing and long-range PCR.

FIREFLOWER

Fire Flower size selection protocol removes shorter DNA fragments from an extracted starting sample.

Fire Flower’s friendly spin-column size-selection protocol option that uses some of the Fire Monkey kit components. This size-selection protocol removes many of the smaller DNA fragments and can be performed on any DNA sample regardless of the extraction method used to produce the sample. Fire Flower is a 15 minute process that depletes DNA fragments under 10kb and improves the molecular ratio of any sample.

The product of a Fire Flower size selection procedure can be processed by any sequencing technology.

Automation

RevoluGen has developed and validated an automated protocol for Fire Monkey/Fire Flower. The automated protocal uses a multi-well filter plate format that enables high volume throughput and multiplexed hands-free HMW-DNA extraction. The commercial launch of this automated Fire Monkey/ Fire Flower is anticipated soon. To become a test site or to discuss automated applications, get in touch