|Product name||ExKine™ Mitochondrion Extraction Kit (Cultured Cells)|
|Applications notes||Abbkine ExKine™ Mitochondrion Extraction Kit (Cultured Cells) enables rapid and crude isolation of of intact mitochondria from cultured mammalian cells. The kit offers two protocols for the separation of mitochondria from cytosolic components relying on differential centrifugation. First protocol utilizes a reagent-based method allowing multiple samples to be processed concurrently. The second protocol uses traditional Dounce homogenization and provides approximately two-fold more mitochondria.|
|Kit components||• Lysis Buffer A (5×)
• Lysis Buffer B
• Lysis Buffer C
• Storage Buffer
|Features & Benefits||• Isolate intact mitochondria from 20 million cells in less than 1 hour.
• Process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method.
• Isolated mitochondria is compatible with almost any downstream application.
|Usage notes||Perform all steps at 2–8 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.|
|Storage instructions||Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Mitochondria is a double-membrane-bound organelle found in most eukaryotic cells. The function of mitochondria is to provide cellular energy. Moreover, mitochondria are involved in other tasks, such as signaling, cellular differentiation, and cell death, as well as maintaining control of the cell cycle and cell growth. Mitochondria have been implicated in several human diseases, including mitochondrial disorders, cardiacdysfunction, heart failure and autism. Mitochondria may play an important role in these cellular processes.|
Fig. 293T cells were extracted with ExKine™ Mitochondrion Extraction Kit (Cultured Cells). Mitochondrial (M) and Cytosolic (C) fractions were analyzed by WB using cytochrome c antibody (cat#ABM40191), VDAC1 antibody (cat#ABP53121) and COX IV antibody (cat#A01060).