Exosome Purification and RNA Isolation Kits, Norgen Biotek, Canada

Intact Exosome Purification

Purification and enrichment of intact plasma/serum, urine, cell culture media and saliva exosomes
Purified exosomes for use in functional studies prepared in 30 minutes
Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
No time-consuming ultracentrifugation, filtration or special syringes required
No precipitation reagents, overnight incubation, protease or coagulant treatments required
Purified exosomes are free from any protein-bound cell-free circulating RNA
Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon-Carbide resin separation matrix

Purification and enrichment of intact exosomes from plasma/serum, urine and cell culture media followed by subsequent exosomal RNA isolation from the purified intact exosomes
Purified exosomes for use in functional studies prepared in 30 minutes
Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA, irrespective of size or GC content, without bias
Purify high-quality RNA in 15-20 minutes
Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL
The purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon-Carbide resin separation matrix

Purification and enrichment of intact exosomes from plasma/serum and urine followed by subsequent RNA isolation from both the purified intact exosomes and the circulating cell-free RNA
Purified exosomes for use in functional studies prepared in 30 minutes
Purify high-quality exosomal and cell-free circulating RNA (cfc-RNA) in 15-20 minutes
Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type
The purified exosomes are free from any protein-bound cell-free circulating RNA
Sequential isolation of all sizes of exosomal/extracellular vesicle RNA and cfc-RNA, including microRNA in two separate fractions irrespective of size or GC content, without bias
Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL
The purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration
The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon-Carbide resin separation matrix

Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA, from previously purified exosomes
Bind and elute all RNA irrespective of size or GC content, without bias
No phenol extractions, Proteinase K treatment or carrier RNA required
Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL
Purify high-quality RNA in 15-20 minutes
Compatible with exosome and extracellular vesicles purified using Norgen’s exosome purification technology or using any other exosome purification technology such as ultracentrifugation, Exoquick, filtration or others
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon-Carbide resin separation matrix

Efficient depletion of cow’s exosomes from Fetal Bovine Serum or any other serum source prior to use as a growth supplement in culture medium
Deplete exosome-sized vesicles from versatile serum volumes of up to 280 mL
No protease treatment required
No time-consuming ultracentrifugation
No precipitation reagents or overnight incubation required
Depleted FBS has no detectable cow’s miRNA
The depleted FBS provides the same cellular growth rates as the standard FBS
Purification is based on spin column chromatography that uses Norgen’s proprietary Silicon-Carbide resin separation matrix