FastPure EndoFree Plasmid Maxi Kit is specially designed for the purification of endotoxin-free plasmid DNA from 150 ml – 300 ml of overnight
bacterial cultures. Based on an optimized SDS alkaline lysis technology, the endotoxin in crude extract selectively binds to a unique Endotoxin
Removal Buffer and then removed by centrifugation, while the plasmid DNA selectively binds to the silicon membrane in the spin column under
high-salt and low-pH condition. Then, the RNA, proteins, and other impurities are removed by the wash buffer. Finally, the plasmid DNA is eluted
from silicon membrane in a low-salt concentration of buffer with high-pH. This kit needs no phenol, chloroform, or other reagents that are toxic,
and needs no ethanol precipitation. Pure high-copy plasmid DNA of 0.2 mg – 1.5 mg can be quickly extracted with a yield rate of 80% – 90% and
a endotoxin level of < 0.1 EU/μg plasmid DNA. The plasmid DNA obtained can be used for high-efficient cell transfection, and can also be used
in a wide variety of molecular biology procedures, including restriction endonuclease digestion, PCR, in vitro transcription, transformation, and
Applicable for large scale isolation of high-quality endotoxin-free plasmid DNA from 150 ml – 300 ml of overnight cultures.
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