SafeSeqTM DNA tube, BIOENTIST, THAILAND

SafeSeq^TM DNA tube

DNA Transportation Tube, for NGS

Product specification

The SafeSeq^TM DNA tube is supplied as coating DNA preservative agents at the bottom of each tube. The SafeSeq^TM DNA tube is suitable for preservation of DNA (ranging from 0.065 to 40 µg) at room temperature up to 1 month (more than 88% of DNA recovery rate) [Figure 1]. In addition, the SafeSeq^TM DNA tube is compatible with DNA extracted from standard protocols of commercially available DNA extraction kits as well as the manual phenol-chloroform extraction. Moreover, the SafeSeq^TM DNA tube can be applied for preservation of various forms (circular double stranded DNA, linear double stranded DNA and linear single stranded DNA) and several sources (Human, Bacteria and Fungus) of DNA [Figure 2]. The DNA recovered from the SafeSeq^TM DNA tube is highly intact without degradation [Figure 3]. In summary, the SafeSeq^TM DNA tube is the best solution for shipping and delivery of DNA at room temperature.

Storage and transportation

The SafeSeq^TM tube should be stored and transported at room temperature. The shelf-life of SafeSeq^TM DNA tube is at least 3 months.

Simplified workflow

1. Apply DNA into the SafeSeq^TM DNA tube
2. Dry DNA in the SafeSeq^TM DNA tube
3. DNA recovery from the SafeSeq^TM DNA tube
4. DNA is ready to use in downstream molecular application

SafeSeq^TM DNA tube protocols

Apply DNA into the SafeSeq^TM DNA tube

1. Add 65 ng – 40 µg of DNA in a final volume of 10-100 µl into the bottom of the SafeSeq^TM DNA tube
2. Mix by pipetting to ensure that the DNA is well mixed with the DNA preservative agents
3. Proceed to DNA drying protocol

Dry DNA in SafeSeq^TM DNA tube

4. Dry DNA in SafeSeq^TM DNA tubes with caps off, according to either of the methods described below

• Vacuum: approximately 1 hour/10 µl of DNA solution
• SpeedVac^®: 2 – 4 hours
• Under Biosafety cabinet: 14 hours

5. When DNA is completely dried, cap the tubes and transport SafeSeq^TM DNA tubes at room temperature.

DNA recovery from SafeSeq^TM DNA tube

6. Add nuclease-free water (equivalent to the input volume)
7. Mix by pipetting up and down 10 times to solubilize the DNA at room temperature (Do not attempt to recover DNA on ice)
8. Cap the tube, gently mix by flicking the tube 10 times and briefly centrifuge.
9. DNA is ready to use in downstream molecular applications

     SafeSeq^TM DNA tube

      DNA Transportation Tube, for Next-Generation

      Sequencing (NGS)

• Ready & Easy -to-use
• Best for transportation from lab-to-lab or import/export
• Safe DNA quality at Room Temperature up to 30 days

Summary of product specification

Protocol_of_SafeSeq-DNA_tube