Workflow for cell capture, extraction and reverse purification:
- mix sample with Buffer EN and Beads B
- incubate for 3 minutes at ambient temperature
- place tubes in a magnetic rack
- discard supernatant
- wash Beads B once with Buffer EN (could be obmitted, depending on the specimen type)
- resuspend Beads B in Buffer DL
- incubate for 5 to 15 minutes at 95°C
- remove tubes from heat block and place in a magnetic rack
- use supernatant for downstream application
Validated for the following sample types:
- Swine oral fluid
- Oral and nasal swabs in transport medium
- Environmental swabs in water or saline
- Urine
- Saliva