MolYsis, Molzym, Germany
Pathogen DNA Enrichment and Isolation
MolYsis™ kits are ideal for the supply of enriched microbial DNA for the direct analysis of pathogens in human and animal samples. The kits are evaluated for all sorts of body fluids like blood, CSF, synovial aspirates, BAL and other specimens.
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MolYsis™ is a unique system of targeted isolation of microbial DNA from complex clinical samples such as blood, aspirates and other body fluids. The selective lysis of host cells and the degradation of the released host DNA avoid unspecific primer binding and thereby greatly enhance the sensitivity and specificity of PCR or Real-Time PCR analysis of pathogens. For detailed information see Technology. The kit series allows sample processing in the range of 0.2 to 1 ml or 5 ml.
Molzym offers a pre-treatment MolYsis™ Basic kit for host DNA depletion that can be combined with any microbial DNA isolation kit established in the laboratory, including manual and automated systems.
The MolYsis™ Complete kit series provides protocols for the whole process, ranging from pre-treatment for host DNA depletion to microbial DNA isolation.
The MolYsis™ technology is also applicable for tissue and swab samples. With the Ultra-Deep Microbiome Prep kit ≤1 ml liquid and tissue specimens can be used. Highest flexibility is given with the Ultra-Deep Microbiome Prep10 kit for volumes from 1-10 ml and tissues.
All kit ingredients, including buffers, reagents and consumables, are quality-checked for the absence of microbial DNA contaminations. This secures precise molecular analysis.
Automation: The process of microbial DNA enrichment and isolation is also available semi- and fully automated. Click here for more information.
Order information:
| Kits | Sample | Sample Volume | Order No. | Quotation Request |
| Host DNA depletion | ||||
| MolYsis™ Basic5 | liquid biopsy | 0.2-1 ml or 5 ml | D-301-050, D-301-100 | Request form |
| Host DNA depletion and microbial DNA isolation | ||||
| MolYsis™ Complete5 | liquid biopsy | 0.2-1 ml or 5 ml | D-321-050, D-321-100 | |
| Host DNA depletion and microbial DNA isolation from liquid and tissue specimens | ||||
| Ultra-Deep Microbiome Prep | liquid biopsy and tissue | ≤1 ml and tissue | G-020-025, G-020-050 | |
| Ultra-Deep Microbiome Prep10 | liquid biopsy and tissue | 1-10 ml and tissue | G-030-025, G-030-050 | |
Please inquire for more detailed information, applications and references at [email protected] or call at +49 (0) 421 / 69 61 62 15 or visit our Blog.
Download open list of microorganisms found in clinical and other specimens by sequencing
Selected references:
Krohn S , Zeller K, Böhm S, Chatzinotas A, Harms H, Hartmann J, Heidtmann A, Herber A, Kaiser T, Treuheit M, Hoffmeister A, Berg T, Engelmann C (2018) Molecular quantification and differentiation of Candida species in biological pecimens of patients with liver cirrhosis. PLOS One 13(6), e0197319. https://www.ncbi.nlm.nih.gov/pubmed/29897895
Schmidt K, Mwaigwisya S, Crossman LC, Doumith M, Munroe D, Pires C, Khan AM, Woodford N, Saunders NJ, Wain J, O’Grady J, Livermore DM (2017) Identification of bacterial pathogens and antimicrobial resistance directly from clinical urines by nanopore-based metagenomic sequencing. J Antimicrob Chemother 2017; 72: 104–114. https://doi.org/10.1093/jac/dkw397
Thoendel M, Jeraldo PR, Greenwood-Quaintance KE, Yao JZ, Chia N, Hanssen AD, Abdel MP, Patel R (2016) Comparison of microbial DNA enrichment tools for metagenomic whole genome sequencing. Journal of Microbiological Methods 127 (2016) 141–145. http://dx.doi.org/10.1016/j.mimet.2016.05.022
