MALBAC® Platinum Single Cell RNA Amplification Kit specifically performs reverse transcription for mRNA in single cell or few cells, and uses patented MALBAC® Template-switching technology to add adapter containing a quantitative molecular tag to the 3′ end of the cDNA, by which the 3′ amplification preference and the contamination of genomic DNA and rRNA in the cDNA synthesis process are effectively avoided, so that the gene expression file is accurately retained and quantified.
Full-length transcriptComplete transcript amplification, including UTR region and UTR upstream and downstream regions, enabling full-length transcriptome sequencing
Added UMIabsolute quantification of gene expression by UMIs
High sensitivityHigh gene detection rate, which can analyze genes with low expression
Low starting template volume requiredSingle cell RNA or trace RNA (10 pg of RNA in total)
High amplification efficiency2-50 ng high-quality full-length cDNA obtained from trace mRNA after reverse transcription and amplification
ConvenientRapid Procedure with 1 tube,3 steps and 5-6 hours