Get everything you need for nucleic acid extraction and get started right away. Easy – and affordable – automated cleanups.
Nucleic acid extraction using SPRI (solid-phase reversible immobilization) magnetic beads are one of the most common and painstaking processes done by modern molecular biology labs.
Anytime a PCR product is used in sensitive downstream processes like gene assembly or next-generation sequencing (NGS), labs must go through multiple, time- and labor-intensive bead washing steps. The Opentrons Nucleic Acid Extraction Workstation is designed to automate your bead cleanups for you.
This workstation includes everything you need to start automating SPRI bead cleanups in your lab: the robot with magnetic module, correct labware, beads from Omega Bio-tek, and a validated protocol free to customize and download.
This workflow can be used for the following applications:
Accuracy: 15% at 1uL
Precision: 5% at 1uL
Throughput: Fast fill a 96-well plate in 22 seconds
Supports Ethernet or USB connection (external dongle provided)
4 USB ports available to support connection to multiple modules
Easy access to electronics via external PCBA enclosure
LED indicator provide users feedback, diagnosis & troubleshooting aid on instrument power state, system processing, ethernet, and network connectivity
Self-serve unboxing and installation
Reagent and bead system agnostic
Plug-and-play setup with access to protocol libraries to enable immediate assay implementation
Easily integrates to your existing instruments
Compatible with ANSI/SBS compliant or automation friendly tubes, microplates, deep-well plates, reservoirs and adapters
OT-2 Liquid Handling Robot
Magnetic Module
Omega Mag-Bind TotalPure NGS Kit, 5ml
Opentrons Tips
Single-Channel Pipette
8-Channel Pipette
Required Plates and Tube
Simply load your reagents, hit ‘run,’ and let the robot do your cleanups.
Closeup of beads pelleting on the OT-2 magnetic module
This application has been fully tested and scientifically verified with reagent partners Swift Biosciences and Omega Bio-tek.
View our technical note outlining the validation process here.
You can also use any SPRI beads you already have using this generic protocol.
Purification of 20 µL of indexed 16S Metagenomic and ladder samples with 1X and 1.8X bead volumes to show OT-2 size selection on Agilent TapeStation High Sensitivity D1000 ScreenTape®
STEP 1
Select your protocol
Input sample number and desired bead volumes, and download your validated protocol
STEP 2
Calibrate & activate protocol
Put magnetic beads into 2ml tube or 96-well plates and load them on the OT-2 robot
STEP 3
Activate protocol and hit “run”
Let the robot take care of your cleanups for you
STEP 4
Collect purified sample
Collect your clean nucleic acids and continue your experiment
Reagents
This application has been fully tested and scientifically validated in our lab with reagent partner Omega Bio-Tek. We recommend you use the Omega Bio-Tek Total-Pure NGS kit for this application. If you would like to use another kit, the OT-2 supports various kits for nucleic acid extraction.
Labware
This application has been verified with the Opentrons Cleanup Labware Bundle, which includes plates for the magnetic module, reagent reservoirs, and tubes. If you have different labware requirements, reach out to our sales team to discuss modification of this protocol to fit your needs.
Pipettes
This application uses the p300 single-channel or 8-channel pipettes for the OT-2 robot. If you have different volume requirements, contact our sales team to ask about protocol modification.
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